Vaccination accelerates hepatic erythroblastosis induced by blood-stage malaria.

ABSTRACT:

Background

Vaccination induces survival of otherwise lethal blood-stage infections of the experimental malaria Plasmodium chabaudi. Blood-stage malaria induces extramedullary erythropoiesis in the liver. This study investigates how vaccination affects the course of malaria-induced expression of erythrocytic genes in the liver.

Methods

Female Balb/c mice were vaccinated at week 3 and week 1 before challenging with 10⁶ P. chabaudi-parasitized erythrocytes. The non-infectious vaccine consisted of erythrocyte ghosts isolated from P. chabaudi-infected erythrocytes. Gene expression microarrays and quantitative real-time PCR were used to compare mRNA expression of different erythrocytic genes in the liver of vaccination-protected and non-protected mice during infections

SUBMITTER: Delic D 

PROVIDER: S-EPMC6988251 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature