Pre-emptive genomic surveillance of emerging ebolaviruses.

ABSTRACT: Genomic surveillance during ebolavirus outbreaks to elucidate transmission chains and develop diagnostic tests is delayed by the laborious development of variant-specific laboratory assays. We developed a new protocol combining 31 parallel PCR assays with Illumina/MinION-based sequencing, allowing generic ebolavirus genomic surveillance, validated using cell culture-derived Ebola, Reston, Sudan and Taï Forest virus at concentrations compatible with patient viral loads. Our approach enables pre-emptive genomic surveillance of ongoing and future ebolavirus outbreaks irrespective of variant divergence.

SUBMITTER: Postigo-Hidalgo I 

PROVIDER: S-EPMC6988270 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature