TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1.

ABSTRACT:

Background

Tripartite motif-containing proteins (TRIM) play a crucial role in carcinogenesis. Little attention has been focused on the possible functions of TRIM6 on carcinogenesis.

Methods

The expression levels of TRIM6 were assessed in colorectal cancer (CRC) samples. TRIM6 expression was knocked down in CRC cell lines, and subjected to Cell counting kit-8 (CCK-8), bromodeoxyuridine (BrdU) incorporation and cell cycle assays. Immunoprecipitation and proteomics analysis was performed to identify potential associated proteins of TRIM6.

Results

TRIM6 expression was up-regulated in CRC samples and TRIM6 expression may be an independent prognostic marker for CRC. Knocking down TRIM6 expression suppressed CRC cell proliferation, induced cell cycle arrested at G2/M phase a

SUBMITTER: Zheng S 

PROVIDER: S-EPMC6988281 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature