Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection.

ABSTRACT: BACKGROUND:Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30-50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics. METHODS:The recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method that has been used for the detection of several pathogens. The assays were performed in a one-step single tube reaction at 39° Celsius within 15-30 min. In this study, we established an RAA assay for M. pneumoniae using clinical specimens for validation and commercial real-time PCR as the reference method. RESULTS:The analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with an

SUBMITTER: Xue G 

PROVIDER: S-EPMC6988361 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature