Polymorphic markers for identification of parasite population in Plasmodium malariae.

ABSTRACT:

Background

Molecular genotyping in Plasmodium serves many aims including providing tools for studying parasite population genetics and distinguishing recrudescence from reinfection. Microsatellite typing, insertion-deletion (INDEL) and single nucleotide polymorphisms is used for genotyping, but only limited information is available for Plasmodium malariae, an important human malaria species. This study aimed to provide a set of genetic markers to facilitate the study of P. malariae population genetics.

Methods

Markers for microsatellite genotyping and pmmsp1 gene polymorphisms were developed and validated in symptomatic P. malariae field isolates from Myanmar (N = 37). Fragment analysis was used to determine allele sizes at each locus to calculate multiplicity of infections

SUBMITTER: Mathema VB 

PROVIDER: S-EPMC6988369 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature