A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila.

ABSTRACT: The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics. Recently, the Drosophila transgenic technology has been improved by the highly efficient phiC31 site-specific integration system. Many new and improved fluorescent proteins with desirable advantages have been developed. However, the phiC31 system and the newly developed fluorescent proteins have not been systematically applied in Drosophila transgenic vectors. Here, we have constructed a modular toolset of C-terminal fluorescent protein fusion vectors based on phiC31 site-specific integration system for the generation of transgenic Drosophila lines. These cloning vectors contain a variety o

SUBMITTER: Luo J 

PROVIDER: S-EPMC6988884 | biostudies-literature | 2019 Mar - Dec

REPOSITORIES: biostudies-literature