An ELISA based on soluble egg antigens for the serodiagnosis of animal schistosomiasis turkestanica.

ABSTRACT: BACKGROUND:The existing diagnostic techniques for detecting schistosomiasis turkestanica, such as aetiological assays, identify infection by parasitic worms via the incubation of miracidia from faeces or observing eggs under microscopy. However, they are limited in the diagnosis of low-grade and prepatent infections, which lead to a high misdetection rates. Therefore, a new method for parasite diagnosis with increased sensitivity is urgently needed. METHODS:Goats in Nimu County (Tibet, China) infected with Schistosoma turkestanicum in an epidemic area were selected according positivity for the infection by faecal examination. Adult worms were collected, eggs were extracted by the sodium hydroxide (NaOH) erosion method, and soluble worm antigen preparation (SWAP) and soluble egg antigen (SE

SUBMITTER: Ji R 

PROVIDER: S-EPMC6988949 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature