Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue.

ABSTRACT: Recent studies have highlighted the critical role of angiogenesis and sympathetic innervation in adipose tissue remodeling during the development of obesity. Therefore, developing an easy and efficient method to document the dynamic changes in adipose tissue is necessary. Here, we describe a modified immunofluorescent approach that efficiently co-stains blood vessels and nerve fibers in adipose tissues. Compared to traditional and recently developed methods, our approach is relatively easy to follow and more efficient in labeling the blood vessels and nerve fibers with higher densities and less background. Moreover, the higher resolution of the images further allows us to accurately measure the area of the vessels, amount of branching, and length of the fibers by open source software. As a

SUBMITTER: Li X 

PROVIDER: S-EPMC6989151 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature