The Mechanism of Bidirectional pH Taxis in Bacillus subtilis.

ABSTRACT: We investigated pH taxis in Bacillus subtilis This bacterium was found to perform bidirectional taxis in response to external pH gradients, enabling it to preferentially migrate to neutral environments. We next investigated the chemoreceptors involved in sensing pH gradients. We identified four chemoreceptors involved in sensing pH: McpA and TlpA for sensing acidic environments and McpB and TlpB for sensing alkaline ones. In addition, TlpA was found to also weakly sense alkaline environments. By analyzing chimeras between McpA and TlpB, the principal acid- and base-sensing chemoreceptors, we identified four critical amino acid residues-Thr¹⁹⁹, Gln²⁰⁰, His²⁷³, and Glu²⁷⁴ on McpA and Lys¹⁹⁹, Glu²⁰⁰, Gln²⁷³, a

SUBMITTER: Tohidifar P 

PROVIDER: S-EPMC6989800 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature