Functional contribution of sphingosine-1-phosphate to airway pathology in cigarette smoke-exposed mice.

ABSTRACT:

Background and purpose

A critical role for sphingosine kinase/sphingosine-1-phosphate (S1P) pathway in the control of airway function has been demonstrated in respiratory diseases. Here, we address S1P contribution in a mouse model of mild chronic obstructive pulmonary disease (COPD).

Experimental approach

C57BL/6J mice have been exposed to room air or cigarette smoke up to 11 months and killed at different time points. Functional and molecular studies have been performed.

Key results

Cigarette smoke caused emphysematous changes throughout the lung parenchyma coupled to a progressive collagen deposition in both peribronchiolar and peribronchial areas. The high and low airways showed an increased reactivity to cholinergic stimulation and α-smooth muscle actin overexpre

SUBMITTER: De Cunto G 

PROVIDER: S-EPMC6989954 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature