Required G_K1 to Suppress Automaticity of iPSC-CMs Depends Strongly on I_K1 Model Structure.

ABSTRACT: Human-induced pluripotent stem cells derived cardiomyocytes (hiPSC-CMs) are a virtually endless source of human cardiomyocytes that may become a great tool for safety pharmacology; however, their electrical phenotype is immature: they show spontaneous action potentials (APs) and an unstable and depolarized resting membrane potential (RMP) because of lack of I_K1. Such immaturity hampers their application in assessing drug safety. The electronic overexpression of I_K1 (e.g., through the dynamic clamp (DC) technique) is an option to overcome this deficit. In this computational study, we aim to estimate how much I_K1 is needed to bring hiPSC-CMs to a stable and hyperpolarized RMP and which mathematical description of I_K1 is most suitable for DC experim

SUBMITTER: Fabbri A 

PROVIDER: S-EPMC6990378 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature