Quasimetagenomic source tracking of Listeria monocytogenes from naturally contaminated ice cream.

ABSTRACT:

Background

The more quickly bacterial pathogens responsible for foodborne illness outbreaks can be linked to a vehicle of transmission or a source, the more illnesses can be prevented. Whole genome sequencing (WGS) based approaches to source tracking have greatly increased the speed and resolution with which public health response can pinpoint the vehicle and source of outbreaks. Traditionally, WGS approaches have focused on the culture of an individual isolate before proceeding to DNA extraction and sequencing. For Listeria monocytogenes (Lm), generation of an individual isolate for sequencing typically takes about 6 days. Here we demonstrate that a hybrid, "quasimetagenomic" approach ie; direct sequencing of microbiological enrichments (first step in pathogen detection and recove

SUBMITTER: Ottesen A 

PROVIDER: S-EPMC6990534 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature