Silencing of microRNA-517a induces oxidative stress injury in melanoma cells via inactivation of the JNK signaling pathway by upregulating CDKN1C.

ABSTRACT:

Background

Melanoma is notoriously resistant to current treatments, and less than 25% of metastatic melanoma cases respond to existing therapies. Growing evidence has shown that microRNAs (miRNAs) play a vital role in the prognosis of melanoma. MiR-517a has been implicated in many types of cancer; however, its expressional features and potential biological functions in melanoma remain unclear. The present study aimed to investigate the possible effects of miR-517a on oxidative stress (OS) in melanoma cells.

Methods

miR-517a expression in melanoma was determined using RT-qPCR. After treatment with different concentrations of H₂O₂, cell viability was determined in order to identify the most appropriate H₂O₂ concentration. Through los

SUBMITTER: Yang C 

PROVIDER: S-EPMC6990552 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature