Monitoring and external control of pH in microfluidic droplets during microbial culturing.

ABSTRACT: BACKGROUND:Cell-based experimentation in microfluidic droplets is becoming increasingly popular among biotechnologists and microbiologists, since inherent characteristics of droplets allow high throughput at low cost and space investment. The range of applications for droplet assays is expanding from single cell analysis toward complex cell-cell incubation and interaction studies. As a result of cellular metabolism in these setups, relevant physicochemical alterations frequently occur before functional assays are conducted. However, to use droplets as truly miniaturized bioreactors, parameters like pH and oxygen availability should be controlled similar to large-scale fermentation to ensure reliable research. RESULTS:Here, we introduce a comprehensive strategy to monitor and control pH for

SUBMITTER: Tovar M 

PROVIDER: S-EPMC6990587 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature