Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5.

ABSTRACT: BACKGROUND:Necroptosis is a form of programmed cell death that is accompanied by release of intracellular contents, and reportedly contributes to various diseases. Here, we investigate the significance of necroptosis in pancreatic cancer. METHODS:We used immunohistochemistry and western blot analysis to evaluate expression of the key mediators of necroptosis-receptor-interacting serine/threonine protein kinase 3 (RIP3) and mixed lineage kinase domain-like (MLKL)-in human pancreatic cancer. We also tested the effects of conditioned media (CM) from necroptotic cells on pancreatic cancer cells in Transwell migration and Matrigel invasion assays. Protein array analysis was used to investigate possible mediators derived from necroptotic cells. RESULTS:RIP3 and MLKL are highly expressed in human

SUBMITTER: Ando Y 

PROVIDER: S-EPMC6991976 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature