Awake chronic mouse model of targeted pial vessel occlusion via photothrombosis.

ABSTRACT: Animal models of stroke are used extensively to study the mechanisms involved in the acute and chronic phases of recovery following stroke. A translatable animal model that closely mimics the mechanisms of a human stroke is essential in understanding recovery processes as well as developing therapies that improve functional outcomes. We describe a photothrombosis stroke model that is capable of targeting a single distal pial branch of the middle cerebral artery with minimal damage to the surrounding parenchyma in awake head-fixed mice. Mice are implanted with chronic cranial windows above one hemisphere of the brain that allow optical access to study recovery mechanisms for over a month following occlusion. Additionally, we study the effect of laser spot size used for occlusion and demonst

SUBMITTER: Sunil S 

PROVIDER: S-EPMC6992450 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature