Analytical and preparative separation of phosphorothioated oligonucleotides: columns and ion-pair reagents.

ABSTRACT: Oligonucleotide drugs represent an emerging area in the pharmaceutical industry. Solid-phase synthesis generates many structurally closely related impurities, making efficient separation systems for purification and analysis a key challenge during pharmaceutical drug development. To increase the fundamental understanding of the important preparative separation step, mass-overloaded injections of a fully phosphorothioated 16mer, i.e., deoxythymidine oligonucleotide, were performed on a C18 and a phenyl column. The narrowest elution profiles were obtained using the phenyl column, and the 16mer could be collected with high purity and yield on both columns. The most likely contribution to the successful purification was the quantifiable displacement of the early-eluting shortmers on both colum

SUBMITTER: Enmark M 

PROVIDER: S-EPMC6992550 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature