Exosomal microRNA in peritoneal fluid as a biomarker of peritoneal metastases from gastric cancer.

ABSTRACT:

Aim

Peritoneal metastases (PM) frequently occur in patients with gastric cancer and result in a poor prognosis. Exosomes play pivotal roles in tumor metastasis through the transfer of microRNAs (miRNAs). We examined the exosomal miRNA profile in peritoneal fluids to identify novel biomarkers to reflect tumor burden in the peritoneum.

Methods

Exosomes were isolated from peritoneal fluids of patients of gastric cancer with macroscopic (P1) or microscopic (P0CY1) peritoneal metastasis (PM) and comprehensive miRNA expression analysis was carried out. Expressions of candidate miRNAs were then validated in all 58 samples using TaqMan Advanced miRNA Assays.

Results

In initial screening, we carried out comprehensive analysis of exosomal miRNA using peritoneal fluids from 11 a

SUBMITTER: Ohzawa H 

PROVIDER: S-EPMC6992685 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature