ALKBH5-mediated m⁶A demethylation of lncRNA PVT1 plays an oncogenic role in osteosarcoma.

ABSTRACT:

Background

Osteosarcoma (OS) is one of the most common malignant bone tumors. Plasmacytoma variant translocation 1 (PVT1) is a well-known oncogenic long noncoding RNA (lncRNA). However, to date, the regulatory mechanism of PVT1 upregulation in OS remains unknown.

Methods

qRT-PCR was carried out to test the expression level of PVT1 and ALKBH5. RNA immunoprecipitation (RIP) and RNA pull-down assays were performed to detect the interaction of PVT1 with ALKBH5 and YTHDF2. Methylated RNA immune-precipitation (MeRIP) was used to examine the N ⁶-methyladenosine (m⁶A) modification of PVT1 transcript.

Results

In this study, we found that PVT1 expression was upregulated in OS tissues and cells and significantly related with clinical stage, tumor siz

SUBMITTER: Chen S 

PROVIDER: S-EPMC6993345 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature