Identification of cellular microRNA miR-188-3p with broad-spectrum anti-influenza A virus activity.

ABSTRACT:

Background

Influenza A virus (IAV) continues to pose serious threats to public health. The current prophylaxis and therapeutic interventions for IAV requires frequent changes due to the continuous antigenic drift and antigenic shift of IAV. Emerging evidence indicates that the host microRNAs (miRNAs) play critical roles in intricate host-pathogen interaction networks. Cellular miRNAs may directly target virus to inhibit its infection and be developed as potential anti-virus drugs.

Methods

In this study, we established a broad-spectrum anti-IAV miRNA screening method using miRanda software. The screened miRNAs were further verified by luciferase assay, viral protein expression assay and virus replication assay.

Results

Five cellular miRNAs (miR-188-3p, miR-345-5p, miR-

SUBMITTER: Cui H 

PROVIDER: S-EPMC6993346 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature