Blood signatures for second stage human African trypanosomiasis: a transcriptomic approach.

ABSTRACT:

Background

Rhodesiense sleeping sickness is caused by infection with T. b rhodesiense parasites resulting in an acute disease that is fatal if not treated in time. The aim of this study was to understand the global impact of active T. b rhodesiense infection on the patient's immune response in the early and late stages of the disease.

Methods

RNASeq was carried out on blood and cerebral spinal fluid (CSF) samples obtained from T. b. rhodesiense infected patients. The control samples used were from healthy individuals in the same foci. The Illumina sequenced reads were analysed using the Tuxedo suite pipeline (Tophat, Cufflinks, Cuffmerge, Cuffdiff) and differential expression analysis carried out using the R package DESeq2. The gene enrichment and function annotation analysi

SUBMITTER: Mulindwa J 

PROVIDER: S-EPMC6993467 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature