Identification of Penicillium species by MALDI-TOF MS analysis of spores collected by dielectrophoresis.

ABSTRACT: In matrix-assisted laser-desorption and ionization mass spectrometry, spectral differences are frequently observed using different growth media on agar plates and/or different growth times in culture, which add undesirable analytical variance. In this article, we explore an approach to the above problem based upon the rationale that, while protein expression in fungal mycelium may well vary under different growth conditions, this might not apply to the same extent in fungal spores. To this end, we have exploited the fact that while mycelium is generally anchored to the fungal-growth substrate, some fungi produce physically-isolated spores which, as such, are amenable to manipulation using dielectrophoresis (the translational motion of charged or uncharged matter caused by polarization effe

SUBMITTER: Reeve MA 

PROVIDER: S-EPMC6994048 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature