Structure of the transcription coactivator SAGA.

ABSTRACT: Gene transcription by RNA polymerase II is regulated by activator proteins that recruit the coactivator complexes SAGA (Spt-Ada-Gcn5-acetyltransferase)^1,2 and transcription factor IID (TFIID)^2-4. SAGA is required for all regulated transcription⁵ and is conserved among eukaryotes⁶. SAGA contains four modules^7-9: the activator-binding Tra1 module, the core module, the histone acetyltransferase (HAT) module and the histone deubiquitination (DUB) module. Previous studies provided partial structures^10-14, but the structure of the central core module is unknown. Here we present the cryo-electron microscopy structure of SAGA from the yeast Saccharomyces cerevisiae and resolve the core module at 3.3 Å resolution. The core module con

SUBMITTER: Wang H 

PROVIDER: S-EPMC6994259 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature