Estrogen receptor-α in female skeletal muscle is not required for regulation of muscle insulin sensitivity and mitochondrial regulation.

ABSTRACT:

Objective

Estrogen receptor-α (ERα) is a nuclear receptor family member thought to substantially contribute to the metabolic regulation of skeletal muscle. However, previous mouse models utilized to assess the necessity of ERα signaling in skeletal muscle were confounded by altered developmental programming and/or influenced by secondary effects, making it difficult to assign a causal role for ERα. The objective of this study was to determine the role of skeletal muscle ERα in regulating metabolism in the absence of confounding factors of development.

Methods

A novel mouse model was developed allowing for induced deletion of ERα in adult female skeletal muscle (ERαKO^ism). ERαshRNA was also used to knockdown ERα (ERαKD) in human myotubes cultured from primary human

SUBMITTER: Inigo MR 

PROVIDER: S-EPMC6994285 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature