Development of Murine Systemic Lupus Erythematosus in the Absence of BAFF.

ABSTRACT:

Objective

To determine whether systemic lupus erythematosus (SLE) can develop in the absence of BAFF in an SLE-prone host.

Methods

Starting with C57BL/6 mice that express a human BCL2 transgene (Tg) in their B cells (thereby rendering B cell survival largely independent of BAFF-triggered signals), we introgressed this Tg into NZM 2328 mice genetically deficient in BAFF (NZM.Baff^-/- ) to generate NZM.Baff^-/- .Bcl2^Tg mice. Expression of human Bcl-2 and lymphocyte profiles were assessed by fluorescence-activated cell sorting, and serologic profiles were determined by enzyme-linked immunosorbent assay. Immunofluorescence and histologic analyses were performed to assess renal immunopathologic features in the mice, and clinical disease was assessed

SUBMITTER: Stohl W 

PROVIDER: S-EPMC6994329 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature