Measuring the contribution of γδ T cells to the persistent HIV reservoir.

ABSTRACT:

Objective

To study the contribution of γδ T cells to the persistent HIV reservoir.

Design

Fifteen HIV-seropositive individuals on suppressive ART were included. We performed parallel quantitative viral outgrowth assays (QVOA) of resting CD4 T (rCD4) cells in the presence or absence of γδ T cells.

Methods

Resting αβ+CD4 T cells were magnetically isolated from PBMCs using two different custom cocktails, only one kit contained antibodies to deplete γδ T cells, resulting in two populations: rCD4 cells and rCD4 cells depleted of γδ cells. Frequency of infection was analyzed by QVOA and DNA measurements.

Results

Recovery of replication-competent HIV from cultures of rCD4 cells was similar in 11 individuals despite the presence of γδ T cells. In four donors, HIV recov

SUBMITTER: James KS 

PROVIDER: S-EPMC6994336 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature