Antigen-specific in vitro expansion of factor VIII-specific regulatory T cells induces tolerance in hemophilia A mice.

ABSTRACT:

Background

Following protein replacement therapy, one-third of severe hemophilia A patients develop antibodies to factor VIII (FVIII), which also hinders the efficacy of gene therapy. Regulatory T cells (Tregs) have a naturally suppressive function that potentially reduces the immune response to FVIII therapy. Furthermore, antigen-specific Tregs are functionally much more potent than polyclonal cells. Adoptive transfer of antigen-specific Tregs can effectively suppress anti-FVIII antibody responses.

Objective

Develop a clinically feasible protocol to enrich and expand Tregs specific to FVIII for suppressing anti-FVIII immune responses.

Methods

Regulatory T cells are isolated from FVIII-sensitized mice, sorted on CD25^high markers, and expanded specifically w

SUBMITTER: Smith BM 

PROVIDER: S-EPMC6994379 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature