Sphere-induced reprogramming of RPE cells into dual-potential RPE stem-like cells.

ABSTRACT:

Background

The retinal pigment epithelium (RPE) has the potential to regenerate the entire neuroretina upon retinal injury in amphibians. In contrast, this regenerative capacity has been lost in mammals. The reprogramming of differentiated somatic cells into induced pluripotent stem cells (iPSCs) by viral transduction of exogenous stem cell factors has triggered a revolution in regenerative medicine. However, the risks of potential mutation(s) caused by random viral vector insertion in host genomes and tumor formation in recipients hamper its clinical application. One alternative is to immortalize adult stem cells with limited potential or to partially reprogram differentiated somatic cells into progenitor-like cells through non-integration protocols.

Methods

Sphere-induced

SUBMITTER: Chen F 

PROVIDER: S-EPMC6994567 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature