Multi-platform NMR Study of Pluripotent Stem Cells Unveils Complementary Metabolic Signatures towards Differentiation.

ABSTRACT: Stem cells, poised to revolutionize current medicine, stand as major workhorses for monitoring changes in cell fate. Characterizing metabolic phenotypes is key to monitor in differentiating cells transcriptional and epigenetic shifts at a functional level and provides a non-genetic means to control cell specification. Expanding the arsenal of analytical tools for metabolic profiling of cell differentiation is therefore of importance. Here, we describe the metabolome of whole pluripotent stem cells (PSCs) using high-resolution magic angle spinning (HR-MAS), a non-destructive approach for Nuclear Magnetic Resonance (NMR) analysis. The integrated ¹H NMR analysis results in detection of metabolites of various groups, including energy metabolites, amino acids, choline derivatives and

SUBMITTER: Elena-Herrmann B 

PROVIDER: S-EPMC6994671 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature