The Quantitative-Phase Dynamics of Apoptosis and Lytic Cell Death.

ABSTRACT: Cell viability and cytotoxicity assays are highly important for drug screening and cytotoxicity tests of antineoplastic or other therapeutic drugs. Even though biochemical-based tests are very helpful to obtain preliminary preview, their results should be confirmed by methods based on direct cell death assessment. In this study, time-dependent changes in quantitative phase-based parameters during cell death were determined and methodology useable for rapid and label-free assessment of direct cell death was introduced. The goal of our study was distinction between apoptosis and primary lytic cell death based on morphologic features. We have distinguished the lytic and non-lytic type of cell death according to their end-point features (Dance of Death typical for apoptosis versus swelling and

SUBMITTER: Vicar T 

PROVIDER: S-EPMC6994697 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature