MiR-155 Overexpression in OT-1 CD8⁺ T Cells Improves Anti-Tumor Activity against Low-Affinity Tumor Antigen.

ABSTRACT: Therapy by adoptive transfer of ex vivo-expanded tumor-infiltrating or genetically modified T cells may lead to impressive clinical responses. However, there is a need to improve in vivo persistence and functionality of the transferred T cells, in particular, to face the highly immunosuppressive environment of solid tumors. Here, we investigate the potential of miR-155, a microRNA known to play an important role in CD8⁺ T cell fitness. We show that forced expression of miR-155 in tumor antigen-specific T cells improves the tumor control of B16 tumors expressing a low-affinity antigen ligand. Importantly, miR-155-transduced T cells exhibit increased proliferation and effector functions associated with a higher glycolytic activity independent of exogenous glucose. Al

SUBMITTER: Monnot GC 

PROVIDER: S-EPMC6994712 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature