Nox2 dependent redox-regulation of microglial response to amyloid-β stimulation and microgliosis in aging.

ABSTRACT: Microglia express constitutively a Nox2 enzyme that is involved in neuroinflammation by the generation of reactive oxygen species (ROS). Amyloid β (Aβ) plays a crucial role in Alzheimer's disease. However, the mechanism of Aβ-induced microglial dysfunction and redox-regulation of microgliosis in aging remains unclear. In this study, we examined Nox2-derived ROS in mediating microglial response to Aβ peptide 1-42 (Aβ₄₂) stimulation in vitro, in aging-associated microgliosis in vivo and in post-mortem human samples. Compared to controls, Aβ₄₂ markedly induced BV2 cell ROS production, Nox2 expression, p47^phox and ERK1/2 phosphorylation, cell proliferation and IL-1β secretion. All these changes could be inhibited to the control levels in the presence of Nox2 in

SUBMITTER: Geng L 

PROVIDER: S-EPMC6994719 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature