Cardioprotective effects of genetically engineered cardiac stem cells by spheroid formation on ischemic cardiomyocytes.

ABSTRACT:

Background

Sca-1+ cardiac stem cells and their limited proliferative potential were major limiting factors for use in various studies.

Methods

Therefore, the effects of sphere genetically engineered cardiac stem cells (S-GECS) inserted with telomerase reverse transcriptase (TERT) were investigated to examine cardiomyocyte survival under hypoxic conditions. GECS was obtained from hTERT-immortalized Sca-1+ cardiac stem cell (CSC) lines, and S-GECS were generated using poly-HEMA.

Results

The optimal conditions for S-GECS was determined to be 1052 GECS cells/mm² and a 48 h culture period to produce spheroids. Compared to adherent-GECS (A-GECS) and S-GECS showed significantly higher mRNA expression of SDF-1α and CXCR4. S-GECS conditioned medium (CM) significantl

SUBMITTER: Jeong HS 

PROVIDER: S-EPMC6995053 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature