Identification of functional regulatory elements in the human genome using pooled CRISPR screens.

ABSTRACT:

Background

Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function. However, protein-coding genes comprise < 2% of the sequence space in the human genome leaving a substantial portion of the genome uninterrogated. Noncoding regions of the genome harbor important regulatory elements (e.g. promoters, enhancers, silencers) that influence cellular processes but high-throughput methods for evaluating their essentiality have yet to be established.

Results

Here, we describe a CRISPR-based screening approach that facilitates the functional profiling of thousands of noncoding regulatory e

SUBMITTER: Borys SM 

PROVIDER: S-EPMC6995077 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature