Basal hsp70 expression levels do not explain adaptive variation of the warm- and cold-climate O_{3 + 4 + 7} and O_ST gene arrangements of Drosophila subobscura.

ABSTRACT:

Background

Drosophila subobscura exhibits a rich inversion polymorphism, with some adaptive inversions showing repeatable spatiotemporal patterns in frequencies related to temperature. Previous studies reported increased basal HSP70 protein levels in homokaryotypic strains for a warm-climate arrangement compared to a cold-climate one. These findings do not match the similar hsp70 genomic organization between arrangements, where gene expression levels are expected to be similar. In order to test this hypothesis and understand the molecular basis for hsp70 expression, we compared basal hsp70 mRNA levels in males and females, and analysed the 5' and 3' regulatory regions of hsp70 genes in warm- and cold-climate isochromosomal O_{3 + 4 + 7} and O_ST lines of D. subobs

SUBMITTER: Puig Giribets M 

PROVIDER: S-EPMC6995229 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature