A microcarrier-based spheroid 3D invasion assay to monitor dynamic cell movement in extracellular matrix.

ABSTRACT:

Background

Cell invasion through extracellular matrix (ECM) is a critical step in tumor metastasis. To study cell invasion in vitro, the internal microenvironment can be simulated via the application of 3D models.

Results

This study presents a method for 3D invasion examination using microcarrier-based spheroids. Cell invasiveness can be evaluated by quantifying cell dispersion in matrices or tracking cell movement through time-lapse imaging. It allows measuring of cell invasion and monitoring of dynamic cell behavior in three dimensions. Here we show different invasive capacities of several cell types using this method. The content and concentration of matrices can influence cell invasion, which should be optimized before large scale experiments. We also introduce further a

SUBMITTER: Liu H 

PROVIDER: S-EPMC6995242 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature