The Plasma Factor XIII Heterotetrameric Complex Structure: Unexpected Unequal Pairing within a Symmetric Complex.

ABSTRACT: Factor XIII (FXIII) is a predominant determinant of clot stability, strength, and composition. Plasma FXIII circulates as a pro-transglutaminase with two catalytic A subunits and two carrier-protective B subunits in a heterotetramer (FXIII-A₂B₂). FXIII-A₂ and -B₂ subunits are synthesized separately and then assembled in plasma. Following proteolytic activation by thrombin and calcium-mediated dissociation of the B subunits, activated FXIII (FXIIIa) covalently cross links fibrin, promoting clot stability. The zymogen and active states of the FXIII-A subunits have been structurally characterized; however, the structure of FXIII-B subunits and the FXIII-A₂B₂ complex have remained elusive. Using integrative hybrid approaches inc

SUBMITTER: Singh S 

PROVIDER: S-EPMC6995596 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature