Manipulation of the tumor microenvironment by cytokine gene transfection enhances dendritic cell-based immunotherapy.

ABSTRACT: The tumor microenvironment strongly influences clinical outcomes of immunotherapy. By transfecting genes of relevant cytokines into tumor cells, we sought to manipulate the microenvironment so as to elicit activation of T helper type 1 (Th1) responses and the maturation of dendritic cells (DCs). Using a synthetic vehicle, the efficiency of in vivo transfection of GFP-cDNA into tumor cells was about 7.5% by intratumoral injection and about 11.5% by intravenous injection. Survival was significantly improved by both intratumoral and intravenous injection of the plasmid containing cDNA of interferon-gamma, followed by intratumoral injection of DCs presenting the tumor antigens. Also, tumor growth was inhibited by these treatments. A more significant effect on survival and tumor growth inhibiti

SUBMITTER: Wijesekera DPH 

PROVIDER: S-EPMC6996313 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature