Phytohormone metabolism in human cells: Cytokinins are taken up and interconverted in HeLa cell culture.

ABSTRACT: Cytokinins (CKs) encompass a group of phytohormones, known to orchestrate many critical processes in plant development. Excluding Archaea, CKs are pervasive among all kingdoms, but much less is reported about their metabolism beyond plants. Recent evidence from mammalian tissues indicates the presence of six additional CK forms beyond the previously identified, single mammalian CK, N⁶-isopentenyladenosine (i⁶A). There is limited understanding of CK biosynthesis pathways in mammalian systems; therefore, human cervical cancer (HeLa) cells were used to further characterize CK processing by tracking the interconversion of CKs into their various structural derivatives in mammalian cells in a time-course study. Through high-performance liquid chromatography-positive electro

SUBMITTER: Aoki MM 

PROVIDER: S-EPMC6996375 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature