EGFR Promotes the Development of Triple Negative Breast Cancer Through JAK/STAT3 Signaling.

ABSTRACT:

Purpose

To investigate the role of EGFR and STAT3 in breast cancer development and progression.

Methods

Through bioinformatics analysis differently expressed genes (DEGs) including EGFR and STAT3 were identified in breast cancer tissue. QRT-PCR and Western blot analysis were used to investigate EGFR and STAT3 levels in breast cancer tissues and cells. The influence of EGFR and STAT3 on the breast cancer cell proliferation (CCK-8 assay, clone formation assays), migration (wound healing assays) and invasion (transwell assays) were investigated. The influence of EGFR on breast cancer in vivo was examined by Nude mouse transplantation tumor experiments and immunohistochemistry (IHC) staining. The effects of EGFR on breast cancer signaling were assessed via Western blot.

Resul

SUBMITTER: Song X 

PROVIDER: S-EPMC6996555 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature