Differential Post-Translational Amino Acid Isomerization Found among Neuropeptides in Aplysia californica.

ABSTRACT: d-Amino acid-containing peptides (DAACPs) make up a class of post-translationally modified peptides in animals that play important roles as cell-to-cell signaling molecules. Despite the functional importance of l- to d-residue isomerization, little is known about its prevalence, mostly due to difficulties associated with detecting differences in peptide stereochemistry. Prior efforts to discover DAACPs have been largely focused on pursuing peptides based on homology to known DAACPs or DAACP-encoding precursors. Here, we used a combination of enzymatic screening, mass spectrometry, and chromatographic analysis to identify novel DAACPs in the central nervous system (CNS) of Aplysia californica. We identified five new DAACPs from the pleurin precursor and three DAACPs from previously u

SUBMITTER: Mast DH 

PROVIDER: S-EPMC6996797 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature