Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone.

ABSTRACT: Tissue immunostaining provides highly specific and reliable detection of proteins of interest within a given tissue. Here we describe a complete and simple protocol to detect protein expression during craniofacial morphogenesis/pathogenesis using mouse craniofacial tissues as examples. The protocol consists of preparation and cryosectioning of tissues, indirect immunofluorescence, image acquisition, and quantification. In addition, a method for preparation and cryosectioning of undecalcified hard tissues for immunostaining is described, using craniofacial tissues and long bones as examples. Those methods are key to determine the protein expression and morphological/anatomical changes in various tissues during craniofacial morphogenesis/pathogenesis. They are also applicable to other tissue

SUBMITTER: Yang J 

PROVIDER: S-EPMC6996940 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature