A genome-wide screen identifies genes that suppress the accumulation of spontaneous mutations in young and aged yeast cells.

ABSTRACT: To ensure proper transmission of genetic information, cells need to preserve and faithfully replicate their genome, and failure to do so leads to genome instability, a hallmark of both cancer and aging. Defects in genes involved in guarding genome stability cause several human progeroid syndromes, and an age-dependent accumulation of mutations has been observed in different organisms, from yeast to mammals. However, it is unclear whether the spontaneous mutation rate changes during aging and whether specific pathways are important for genome maintenance in old cells. We developed a high-throughput replica-pinning approach to screen for genes important to suppress the accumulation of spontaneous mutations during yeast replicative aging. We found 13 known mutation suppression genes, and 31 g

SUBMITTER: Novarina D 

PROVIDER: S-EPMC6996960 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature