A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-alpha in the mouse.

ABSTRACT: Proton Nuclear Magnetic Resonance spectroscopy and Gas Chromatography Mass Spectrometry based metabolomics has been used in conjunction with multivariate statistics to examine the metabolic changes in Caenorhabditis elegans following the deletion of nuclear hormone receptor-49 (nhr-49). Deletion of the receptor produced profound changes in fatty acid metabolism, in particular an increase in the ratio of unsaturated to saturated fatty acids, a decrease in the concentration of glucose and increases in lactate and alanine. Given the proposed functional similarity between nhr-49 and the mammalian peroxisome proliferator-activated receptors (PPARs) these changes were compared with the metabolome of the PPAR-alpha null mouse. The metabolomic approach demonstrated a number of similarities includi

SUBMITTER: Atherton HJ 

PROVIDER: S-EPMC6997030 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature