Two-stage electro-mechanical coupling of a K_V channel in voltage-dependent activation.

ABSTRACT: In voltage-gated potassium (K_V) channels, the voltage-sensing domain (VSD) undergoes sequential activation from the resting state to the intermediate state and activated state to trigger pore opening via electro-mechanical (E-M) coupling. However, the spatial and temporal details underlying E-M coupling remain elusive. Here, utilizing K_V7.1's unique two open states, we report a two-stage E-M coupling mechanism in voltage-dependent gating of K_V7.1 as triggered by VSD activations to the intermediate and then activated state. When the S4 segment transitions to the intermediate state, the hand-like C-terminus of the VSD-pore linker (S4-S5L) interacts with the pore in the same subunit. When S4 then proceeds to the fully-activated state, the elbow-like hinge betw

SUBMITTER: Hou P 

PROVIDER: S-EPMC6997178 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature