Functional characterization of tau class glutathione-S-transferase in rice to provide tolerance against sheath blight disease.

ABSTRACT: Glutathione-S-transferase (GST) is an important defense gene that confers resistance against several abiotic and biotic stresses. The present study identifies a tau class GST in rice (Oryza sativa L.), OsGSTU5 (Os09g20220), which provided tolerance against sheath blight (SB) disease, caused by a necrotrophic fungus, Rhizoctonia solani (RS). Overexpression and knockdown rice transgenic lines of OsGSTU5 were generated and tested for the severity of infection during sheath blight disease. The results obtained after RS infection showed that the lesion cover area and hyphal penetration were more in knockdown line and lesser in the overexpression line. Analysis of reactive oxygen species (ROS) accumulation showed more spots of H₂O₂

SUBMITTER: Tiwari M 

PROVIDER: S-EPMC6997320 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature