Deep Analysis of Residue Constraints (DARC): identifying determinants of protein functional specificity.

ABSTRACT: Protein functional constraints are manifest as superfamily and functional-subgroup conserved residues, and as pairwise correlations. Deep Analysis of Residue Constraints (DARC) aids the visualization of these constraints, characterizes how they correlate with each other and with structure, and estimates statistical significance. This can identify determinants of protein functional specificity, as we illustrate for bacterial DNA clamp loader ATPases. These load ring-shaped sliding clamps onto DNA to keep polymerase attached during replication and contain one δ, three γ, and one δ' AAA+ subunits semi-circularly arranged in the order δ-γ₁-γ₂-γ₃-δ'. Only γ is active, though both γ and δ' functionally influence an adjacent γ subunit. DARC identifies, as function

SUBMITTER: Tondnevis F 

PROVIDER: S-EPMC6997377 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature