Arterial Medial Calcification through Enhanced small Extracellular Vesicle Release in Smooth Muscle-Specific Asah1 Gene Knockout Mice.

ABSTRACT: Arterial medial calcification (AMC) involves an increased small extracellular vesicle (sEV) secretion and apatite calcium precipitation in the arterial wall. The mechanisms mediating AMC remain poorly understood. In the present study, smooth muscle-specific acid ceramidase (Ac) gene knockout mice (Asah1^fl/fl/SM^Cre) were used to demonstrate the role of lysosomal ceramide signaling pathway in AMC. Asah1^fl/fl/SM^Cre mice were found to have more severe AMC in both aorta and coronary arteries compared to their littermates (Asah1^fl/fl/SM^wt and WT/WT mice) after receiving a high dose vitamin D. These mice also had pronounced upregulation of osteopontin and RUNX2 (osteogenic markers), CD63, AnX2 (sEV markers) and ALP expression (mine

SUBMITTER: Bhat OM 

PROVIDER: S-EPMC6997457 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature