Quantification of bile acids: a mass spectrometry platform for studying gut microbe connection to metabolic diseases.

ABSTRACT: Bile acids (BAs) serve multiple biological functions, ranging from the absorption of lipids and fat-soluble vitamins to serving as signaling molecules through the direct activation of dedicated cellular receptors. Synthesized by both host and microbial pathways, BAs are increasingly understood as participating in the regulation of numerous pathways relevant to metabolic diseases, including lipid and glucose metabolism, energy expenditure, and inflammation. Quantitative analyses of BAs in biological matrices can be problematic due to their unusual and diverse physicochemical properties, making optimization of a method that shows good accuracy, precision, efficiency of extraction, and minimized matrix effects across structurally distinct human and murine BAs challenging. Herein we develop an

SUBMITTER: Choucair I 

PROVIDER: S-EPMC6997600 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature