CircRIP2 accelerates bladder cancer progression via miR-1305/Tgf-β2/smad3 pathway.

ABSTRACT:

Background

Increasing evidences indicate that circular RNAs exert critical function in regulating bladder cancer progression. However, the expressive patterns and roles of circular RNAs in bladder cancer remain less investigated.

Methods

circRIP2 was identified and evaluated by RNA-sequencing and qPCR; in vitro effects of circRIP2 were determined by CCK8, clone forming, wound healing and trans-well assays; while mice subcutaneous tumor model was designed for in vivo analysis. Western blot, RNA pulldown assay, miRNA capture and dual luciferase assessment were applied for mechanistic studies.

Results

circRIP2 was identified as a conserved and dramatically repressed circular RNA in bladder cancer. Patients that displayed higher circRIP2 expression negatively associate wi

SUBMITTER: Su Y 

PROVIDER: S-EPMC6998850 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature